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Comparison of the affect of temperature on immobilized lactase, compared with non-immobilized lactase
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Comparison of the affect of temperature on immobilized lactase, compared with non-immobilized lactase Aim: To investigate the effect of temperature on the activity of non-immobilized lactase and the effect of temperature on the activity of immobilized lactase. Introduction Enzymes are substances that act as catalysts; they are biological catalysts, which increase the rate of chemical reactions. Most enzymes are large globular protein molecules with complex 3D shapes. Enzymes have a specific site on their surface, known as the active site, where the substrate, the substance which the enzyme combines with, combines at. The shape of the active site is complementary to the shape of the substrate molecule, to form an enzyme-substrate complex. This process is called the 'lock and key' hypothesis, as the enzymes are specific which means that each enzyme only combines with one type of substrate. It has now also been suggested that the active site is able to change shape in order...
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