Coursework.Info - Inspiration in an Instant

On the left is an image preview of every page of this document, and below are the first 150 words with formatting removed:

Comparing the effect of different temperatures on free lipase and immobilised lipase Plan Lipase is an enzyme which hydrolyses fats to fatty acids and glycerol. Glycerol has three hydroxyl groups. Fatty acid molecules are much larger than glycerol molecules and consist of long hydrocarbon chains. Lipase works by hydrolysis, adding three molecules of water across the ester link. Hypothesis My hypothesis is that immobilised lipase will work more efficiently at high temperatures than free lipase. Free lipase is when lipase is prepared free in solution. It is used to digest fats as, for e.g., in some biological washing powders. It is also used in the food industry. Free lipase works best at temperatures of 30-50°C. However, when an enzyme is immobilised some of its physical properties are changed. There are three ways to immobilise enzymes. Entrapment is the gentlest method of immobilisation and does not damage enzymes. The enzymes are also more stable due to entrapment....

To see the full version of this document, and 145,348 others