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AS level Biology Coursework Cloning Cloning has received a lot of interest recently, with the death of Dolly the sheep, 'the first mammal ever created from the non-reproductive tissue of an adult animal', and whether her death had anything to do with the fact that she was a clone, as well as the current rumours over human clones and the moral issues involved with it. Cloning involves a process called nuclear transfer, which was first explored by Spemann in the 1920's to conduct genetics research. All cloning experiments of adult mammals have used a variation of nuclear transfer. Nuclear transfer requires two cells, a donor cell and an oocyte, or egg cell. Research has proven that the egg cell works best if it is unfertilized, because it is more likely to accept the donor nucleus as its own. The egg cell must be enucleated. The nucleus is removed from the egg cell....

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