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Biology Report: Biology Investigation Maggie Ming F.5Sc (25) Hypothesis: a) Food stored is used up during germination for respiration for energy for growth by cells b) Food stored is consumed by micro-organisms Aim: To test for the presence of amylase in germinating seeds. Principle: a. Starch b. Hydrolysis c. Water + Starch amylase Maltose d. Amylase e. Action of amylase f. Amount of starch used g. Light sensormeter h. Temperature and pH i. A control is necessary. A control can be set up by replacing the fresh enzyme extract from the beans with boiled enzyme extract from the beans. The control set-up differs from the experimental set-up by the enzyme extract. Precautions: (1) Ensure that light from the surroundings will not interfere the results by covering up the 100ml plastic vial with a black cardboard paper. (2) Ensure that, in the control set-up, the bean extract is boiled thoroughly to denature all the enzyme inside. (3) The bean extract should be filter through a few layers of wet muslin cloth...

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