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AS Biology Coursework Investigating the rate of enzyme activity with varying enzyme concentration Aim: To Investigating the Rate of Enzyme Activity with Varying Enzyme Concentration Introduction: Throughout this experiment I will be investigating the effect of enzyme activity whilst varying the enzyme concentration. I will be testing the activity of the enzyme trypsin on the substrate Casein whilst varying the concentration of trypsin. Trypsin is an enzyme that acts to degrade protein; it is referred to as a proteolytic enzyme or proteinase. Trypsin enzyme secreted by the pancreas in most animals, which functions in the digestion of proteins. Pancreatic fluid is introduced into the small intestine through several ducts. It contains trypsin and chymotrypsin, enzymes that split complex proteins into simpler components that can be absorbed and used in reconstructing body proteins. Trypsin is one of the three principle proteinases to break down dietary protein molecules e.g. Casein, to their component peptides and amino acids. Trypsin continues...

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