An investigation to determine how increasing the concentration of inhibitor Copper Sulpahte will affect the amount of juice pr
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An investigation to determine how increasing the concentration of inhibitor Copper Sulpahte will affect the amount of juice produced by pectinase from a given mass of apple Hypothesis: I think that increasing the concentration of the inhibitor copper sulphate will decrease the ammount of juice produced by the enzyme pectinase. I expect this to happen because as copper sulphate is an inhibitor (non-competitive one), it will reduce the break down of pectin found in the middle lamellae between the cell walls in the apple pulp. Enzyme inhibitors are molecules that interact in some way with the enzyme to prevent it from working in the normal manner. In this case, it attaches to a part of the enzyme stucture, other than the active site. When this happens, the shape of the whole enzyme may change, altering the shape of the active site. This prevents enzyme substrate complexes formation, which means the enzyme can...

