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An investigation into the factors that affect the amount of phosphate produced in the reaction between phenolphthalein phosphate and phosphatase enzyme.
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Biology science investigation: an investigation into the factors that affect the amount of phosphate produced in the reaction between phenolphthalein phosphate and phosphatase enzyme Plan For this investigation I will be investigating the factors that affect the amount of phosphate produced in a reaction between phenolphthalein phosphate and phosphatase enzyme. For this I will obviously need the substances for the reaction to take place. I will take the enzyme phosphatase from the tips of mung bean seeds and add them to a phenolphthalein phosphate substrate. This reaction will produce phenolphthalein and phosphate, when the reaction has stopped the phenolphthalein will turn magenta on addition of an alkali. I can measure the strength of the solution by the darkness of the magenta. I will use magenta filters which will help me determine the strength of the solution, by judging the shade of magenta. I will record the readings I take from using the magenta...
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