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An investigation into the effect of varying pH on the yield of apple juice by pectinase.  

Member rating: 7 out of 10 stars (2 votes) | Words: | Submitted: Mon Feb 09 2004

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An investigation into the effect of varying pH on the yield of apple juice by pectinase Hypothesis There will be an optimum pH for pectinase activity. Deviations either side of the optimum will result in decreased pectinase activity. Biological Knowledge Fruits are comprised mostly of unspecialised tissue called parenchyma. The parenchyma cells have thin walls made of two layers. The outermost of these, the primary cell wall, is formed from cellulose fibres called microfibrils surrounded by a matrix of pectins, hemicellulose and proteins. In unripe fruit the pectin is bound to cellulose microfibrils in the cell walls. Such pectin is insoluble and the liquid within the cells remains fluid. During ripening, pectin is partly degraded and solubilised by indigenous pectinases. The result is that the pectin becomes more soluble and its grip on the surrounding cell walls is loosened. One consequence of the partial breakdown of insoluble pectin is that it becomes soluble...

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