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AS-Level Biology Coursework An investigation into the effect of Hydrogen Peroxide concentration on Yeast Catalyse activity Introduction Hydrogen Peroxide (H202) is a by-product of several different metabolic reactions within cells. It is a free radical, and as such can oxidise biological molecules, therefore damaging or killing the cell which produced it. To protect themselves, cells synthesise from amino acids Catalase, an enzyme which catalyses the decomposition of Hydrogen Peroxide into water and oxygen. H202 --> 2H20 + 02 Catalase lowers the activation energy required for this reaction to take place, thus speeding up the rate at which H2O2 is converted to water and oxygen. It does this by having specific active sites; places on the molecule shaped in a way that fits the shape of Hydrogen Peroxide molecules, encouraging them to bind to the enzyme there. This forms an enzyme-substrate complex. Catalase like all enzymes is a globular protein, but as well as its polypeptide chains,...

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