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An immobilised enzyme.
Member rating: No Rating | Words: | Submitted: Mon Mar 01 2004
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An immobilised enzyme is an enzyme literally that has been attached to a support that takes the form of small beads. The techniques for immobilisation vary. 1. Chemical (covalent) attachment of the enzyme to a supporting material. 2. Gel entrapment: the enzyme is mixed with gel-forming ingredients and when the gel forms the enzyme remains "trapped" in the gel matrix. The pores are large enough to let the substrate in, but not the enzyme out. 3. Adsorption to various surfaces is sometimes used but because the attachment is not permanent this method is usually only used for scientific studies or for "disposable" enzymes. 4. Encapsulated in a compartment behind a semi-permeable (porous) membrane 5. Crosslinking of enzyme molecules The immobilised enzyme beads are added into a reaction tank and are recovered later by sedimentation or they are set up in a reactor column so that liquid flows continuously past the...
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