An experiment to show how protease enzymes are affected by temperature

Have a little read: ... Sophie Durham An experiment to show how protease enzymes are affected by temperature Prediction: I think it is likely that this experiment using marvel milk and protease enzyme will react most quickly at 30-40sC. I think this is likely because enzymes work best at the optimum temperature of 37sC, which is the body temperature of human beings. If this is the case, it is also likely that that the enzymes will be denatured at the lower temperature of 12sC and the higher temperatures of 50, 60 and 70sC. This is because the molecules in the enzyme will not vibrate, as they will when the experiment is done at the optimum temperature. Planning- fair test: To make this experiment a fair test the following things have to be constant throughout: * The volume of milk must be at 30ml during the 5 different temperatures ranging from 20sC-70sC * The volume of the enzyme added each time to this milk must be 5ml * The concentrations of the acid and milk each time is going to be: milk 1% and enzyme 0.5% * The temperature of the water in the beakers, when preparing the enzymes and the milk in the boiling tubes is going to be the same. The temperature of the water is going to be checked using a thermometer. * Both the enzymes and the milk will be tested to check they have equilibrated to the same temperature before they are added together * A stopwatch will be used each time for accurate timing of when the solution (enzyme and milk) has reached its end point. * The conditions of the lab and the room temperature also will be constant throughout the experiment Introduction: Enzymes help us in our everyday lives, they can be used in industrial processes such as leather making, bakery, cheese making, washing powder and the making of animal food, but this experiment concentrates on the job of enzymes in the body. In the body enzymes act as biological catalysts which help in the chemical breakdown and the digestion of foods. They react to form chemicals such as fats, carbohydrates and proteins. In this experiment the enzyme used should break the marvel milk (protein) down to form amino acids, similar to what happens in the body Enzymes only work in specific conditions, and this experiment should also demonstrate that the correct temperature is required for the digestion of food in the body. This temperature is 37sC (body temperature) Enzymes must have the correct