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An experiment to investigate the effect of Copper (II) Sulphate on the activity of amylase Introduction Copper (II) Sulphate is an enzyme inhibitor. An enzyme inhibitor is any substance or chemical which if added to the enzyme substrate complex will decrease the rate of reaction. Inhibitors come in two forms: competitive and non-competitive inhibitors. A competitive inhibitor is one that competes for the active site of an enzyme with the enzymes' substrate. It does not affect the enzyme in any way other than occupy the active site making the productivity of the enzyme less efficient. This can be explained clearly in the diagram below: Competitive inhibitors are reversible, that is the inhibitor does not stay bound with the active site but is released again so the enzyme is still able to form the enzyme-substrate complex. It is summarised in the following equation: Enzyme + Inhibitor Enzyme-Inhibitor Complex Competitive inhibitors are always reversible. Non-competitive inhibition occurs when the inhibitor does...

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