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An experiment to investigate the effect of chloride ion concentration on the activity of amylase
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| Submitted: Sun Dec 15 2002
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An experiment to investigate the effect of chloride ion concentration on the activity of amylase PLAN Introduction Amylases are a group of enzymes which hydrolyse starch (A glucose polymer) linked by ?-1,4 and ?-1,6 glycosidic bonds to maltose (a disaccharide). Fungal salivary amylase requires the addition of a cofactor, this is a chemical species that does not directly undergo chemical reaction but is required to maintain the shape the active site or aid the activation of certain molecules. This allows regulation of activity for specific enzymes. Objectives To determine how the concentration of chloride ions affects the rate in which salivary amylase hydrolyses starch Independent variable Chloride ion concentration (sodium chloride) Hypothesis The reaction velocity will increase in direct proportion to concentration of chloride ions present until a point where the graph will plateau and no further increase in rate will be observed. Theory Of Hypothesis Some enzyme molecules can exist in two different states. Either...
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