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An Investigation on the Effect of Inhibitor Concentration on Reactions involving Acid Phosphatase. Katherine Pears CONTENTS Introduction and Hypotheses: Introduction...................................................................Page 3 Pilot Study Summary.......................................................Page 3 Hypotheses...................................................................Page 3 Theory..........................................................................Page 4 Methods: Apparatus List................................................................Page 9 Pilot Study.....................................................................Page 9 Method.......................................................................Page 12 Choice of Techniques....................................................Page 13 Controls......................................................................Page 15 Risk Analysis...............................................................Page 16 Analysis: Data Tables...............................................................Page 17 Graphs........................................................................Page 19 Discussion and Evaluation: Explanation..................................................................Page 22 Error analysis...............................................................Page 22 Limitations...................................................................Page 24 Suggestions for Further Study.......................................Page 24 Appendices Bibliography.................................................................Page 26 An Investigation of Inhibitor Concentration on Reactions involving Acid Phosphatase. INTRODUCTION AND HYPOTHESES Introduction I have decided to investigate the effect of altering the concentration of an inhibitor, on an enzyme catalysed reaction. I will be investigating this with the reaction aided by the enzyme acid phosphatase, in which phenolphthalein and phosphates are released. The inhibitor being used will be sodium dihydrogen phosphate. Pilot studies lead to this idea as they were successful and the most interesting theory was provided using this variable. Pilot Study Summary I carried out a pilot study which involved demonstrating the effect of an inhibitor on the reaction involving the breakdown...

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