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An Investigation into the effect of varying enzyme concentration on the rate of an enzyme controlled reaction.
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| Submitted: Thu Mar 25 2004
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Planning An Investigation into the effect of varying enzyme concentration on the rate of an enzyme controlled reaction The aim of my experiment is to see whether there is a relationship between the enzyme concentration and the initial rate of the reaction. The reaction involves catalase breaking down hydrogen peroxide, which is the substrate. The enzyme is yeast. This is the reaction: 2H2O2(l) --> 2H20(l) + O2(g) Background Knowledge Enzymes are biological catalysts, which are made up from groups of protein molecule. An enzyme has an active site, which has a unique shape (lock) into which only a substrate of the exact same unique shape (key) can fit. When this substrate links into the active site it forms something called an enzyme-substrate complex. Catalysts are substances, which speed up the rate of a reaction, but do not get used up themselves. Enzymes will denature in certain conditions. These conditions are high temperatures and high...
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