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An Investigation into the Effect of Varying pH on Enzyme Activity
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An Investigation into the Effect of Varying pH on Enzyme Activity Introduction Protease is an enzyme that reacts with protein to break it down into its constituents. Enzyme activity can be affected by the change of pH, temperature and the concentration of substrate or enzyme in the solution. The focal aim of this experiment is to see what pH our protease works at best to determine where it is made in the body. The casein used comes from marvel milk and is a protein. It is a protein that is present in milk to give it its white colour. When broken down into amino acids, the milk becomes clearer. When the protease reacts with the casein, it turns clearer from its original cloudy colour. The reaction therefore would be monitored using a colorimeter that measures the amount of light going through the solution and the more light that is let through, the...
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