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An Investigation Into the Effect of Substrate Concentration On the Rate of Enzyme Activity.
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AN INVESTIGATION INTO THE EFFECT OF SUBSTRATE CONCENTRATION ON THE RATE OF ENZYME ACTIVITY INTRODUCTION Hydrogen peroxide is a toxic waste product of respiration. It's conversion to water and oxygen is catalysed by the enzyme CATALASE, which is present in most cells. 2H202 2H20 + 02 hydrogen CATALASE ENZYME water oxygen peroxide gas Hypothesis Increasing the substrate concentration will increase the rate at which hydrogen peroxide is broken down to water and oxygen by the catalase enzyme. Null Hypothesis Increasing the substrate concentration will not affect the rate at which hydrogen peroxide is broken down to water and oxygen by the catalase enzyme. Possible Methods There are a number of possible methods for carrying out this investigation. The easiest way to monitor the reaction is to measure the production of oxygen because it is the gaseous waste product: 1) In this method the reaction takes pace in a test tube. The test tube is connected by a thin glass tube to a...
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