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An Experiment To Show The Effect OF Temperature On The passage Of Red Pigment From a Piece Of Beetroot
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| Submitted: Thu Jul 11 2002
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An Experiment To Show The Effect OF Temperature On The passage Of Red Pigment From a Piece Of Beetroot Apparatus & Materials 1 Cork borer Tripod Beaker Gauze Bunsen Beaker (200-300ml) Thermometer Colorimeter Beetroot Boiling tubes Measuring Cylinder Scalpel Method 1. Using a cork borer cut out 6 cylinders of beetroot. 2. Cut each cylinder to 5cm length. 3. Wash each cylinder for 1 minute. 4. Label 6 boiling tubes A,B,C,D,E,F respectively. 5. Pour 25ml of de-ionised water into boiling tube (A) and place in one beetroot chip. 6. Place the boiling tube in a water bath at 35*c and leave for 10 minutes 7. Using the colorimeter measure the amount of light which passes through the solution in boiling tube A 8. Repeat steps 4-7 for boiling tubes B,C,D,E,F at 45*c, 55*c, 65*c, 75*c and 85*c.respectively. Results Tube Temp % Transmission A 35*c 98% B 45*c 90% C 55*c 73% D 65*c 30% E 75*c 7% F 85*c 3% Conclusion This experiment shows that the pigment from inside the cells of the beetroot passes out more at higher temperatures. The pigment is held in the cell by the cell wall and the...
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