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You are given a cloned gene from Homo sapiens and are asked to identify structurally similar genes (potential orthologues) in a. Arabidopsis. b. Bombyx mori (silk moth). How will you determine the expression pattern of the gene?
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You are given a cloned gene from Homo sapiens and are asked to identify structurally similar genes (potential orthologues) in a. Arabidopsis. b. Bombyx mori (silk moth). How will you determine the expression pattern of the gene? Before analysis of related genes can begin it is important to extract as much information about or cloned gene is possible, as this will aid us in our search for potential orthologues. Firstly I am assuming that the cloned gene is derived from a DNA library and is contained within a vector as part of recombinant DNA. Knowledge about the type of library from which it originated is helpful (either cDNA or Chromosomal) for analysis of the sequence but I will examine that later. The first step is to determine the DNA sequence of this cloned gene. Assuming that I have been given only one colony of the bacteria containing the cloned gene for...
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