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Recombinant DNA is a DNA molecule artificially made from different sources. The different portions are combined into a single molecule. Genetic engineering with recombinant DNA is the re-designing of organisms at the gene level
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Recombinant DNA is a DNA molecule artificially made from different sources. The different portions are combined into a single molecule. Genetic engineering with recombinant DNA is the re-designing of organisms at the gene level with the purpose of achieving specific goals. The first step in genetic engineering usually involves inserting a short piece of 'foreign' DNA into the DNA of a host organism. In a procedure known as gene splicing, a piece of human DNA for example may be inserted into a bacterium. The DNA ring of the bacterial plasmid is broken open to insert the human DNA. The bacterium then acquires the ability to synthesise the protein for which the foreign DNA codes. Gene splicing relies on a group of enzymes called restriction endonucleases. One of these would cut a bacterial plasmid open at a specific site that is determined by the sequence of bases in that region. These bases are a...
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