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Induction of ß-galactosidase Hypothesis and aims By carrying out this experiment I plan to show that in the absence of lactose, the enzyme ß-galactosidase will not be made and the mixture I use to test for this will be colourless. The main aim of the experiment is to demonstrate practically the switching on of a gene. Method To test this hypothesis I will use the bacterium E.Coli which produces the enzyme ß-galactosidase when lactose is present. I will go into the details of how and why later. This is the apparatus, equipment and method I will use. A tick indicates that the named substance will be put into the test tube in question. Test tube 1cm3 E. Coli without lactose 1cm3 E. Coli with lactose 1cm3 Distilled water 1cm3 ß-galacto- sidase 1cm3 ONPG 5 drops Methyl- benzene 5cm3 Sodium Phosphate Colour at start Colour at end 1 ? ? ? ? Colourless 2 ? ? ? ? Colourless 3 ? ? ? ? Colourless 4 ? ? ? ? Yellow The lac operon is the classic example of gene regulation, in which the production of ß-galactosidase (lactase) is induced by the presence of lactose in...

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