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Using Different Carbon Sources For Growth.
Member rating: No Rating | Words: | Submitted: Mon Mar 15 2004
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Using Different Carbon Sources For Growth Introduction: The purpose of this practical is to investigate the ability of yeast to utilize different carbon sources as substrates for respiration. A range of different carbohydrates is used as carbon sources and relatives rates of respiration will be determined by measuring the production of acid by the yeast cells: the faster the rate of respiration, the faster the rate of acid production. Apparatus: * 2% (aqueous solutions of the following sugars: glucose, fructose, galactose, sucrose, lactose, maltose * Top pan balance * Dried yeast granules * Seven 500cm3 flasks, with cotton wool plugs * Ammonium phosphate and ammonium sulphate * Incubator, or water bath, set at 25°C * Burette and stand * 0.1mol dm-3 sodium hydroxide solution * Conical flasks for titration * 25cm3 volumetric pipette and filler * Phenolphthalein indicator solution Method: * Add 200cm3 of each sugar solution to separate, appropriately labelled flasks. Include one flask containing 200cm3 of distilled water as a control. * Add 2g...
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