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Forensic Science Skills For The Laboratory Experiment Title: The Absorption of Light (Calibration Curve) Aims: To establish good safe and organised working practice. To encourage consistent and meticulous keeping of data and records. To develop consistent and accurate laboratory measurement procedures. Background Info: Many substances absorb electromagnetic radiation in the visible or ultraviolet regions of the spectrum as a consequence of electronic transitions within a molecule. Studying such absorptions provides information about the electronic structure of the species. Where absorption occurs in the visible region of the spectrum, the substance is coloured. The colour arises from light of the complementary colour being absorbed, e.g. the purple colour of potassium permanganate is caused by the absorption of green light. At a particular wavelength, the fraction of light absorbed is proportional to the thickness of the absorbing medium (the path length l) and to the concentration of the absorbing species (c): -dI/dl = ?cI (1) Where ? is a constant called the absorption...

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