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Experiment to investigate the the rate of carbohydrate fermentation by Yeast
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| Submitted: Mon Mar 05 2007
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Experiment to investigate the the rate of carbohydrate fermentation by Yeast Aim: To investigate the effect yeast on glucose and other respiratory substrates such as sucrose and starch. I will measure the amount of carbon dioxide bubbles produced using a respirometer. The more bubbles produced in a given time, the higher the rate of respiration. I will achieve this by reacting the yeast (Saccharomyces cerevisiae) with glucose, sucrose and starch at constant temperature, PH, mass and volume. I will keep the temperature constant by using an electronic water bath. This will ensure a fair test because varying temperatures cause varying rates of reaction. I will keep the PH constant by using a buffer solution. This will also ensure a fair test because different PH values alter the active site structure on the yeast enzyme. This will produce inconsistent reaction rates. The mass and volumes will be taken accurately and kept constant...
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