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ColorimetricAnalysis of Glucose.
Member rating: No Rating | Words: | Submitted: Wed Jun 22 2005
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Colorimetric Analysis of Glucose. Aim: To use a colorimetric analysis method and a calibration graph to identify the percentage of glucose in the unknown solutions. Apparatus: * Burette (x 2) * Wooden stand (x 2) * Glucose solution * Distilled water * 100 ml beaker (x 6) * 2 ml syringe * 10 ml syringe * Test tube (x 8) * Benedict's solution reagent * 200 ml beaker * Water bath (Large beaker + Bunsen burner) * Stopwatch * Cuvette * Cotton bud * Funnel (+ Filter paper) * Colorimeter Method: 1. Prepare six glucose solutions of known concentrations, ranging between 0 and 1 %, increasing in 0.2% each time. * To do this, set up the two burettes along with the wooden stands - one burette containing distilled water and the other containing the glucose solution. * In each beaker prepare 5 ml of each glucose solution using the table below as a guideline for the water to glucose ratio. (It is also wise to label each beaker using a marker pen,...
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