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Chromatographic Separation of Carbohydrates.
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Chromatographic Separation of Carbohydrates Thin layer chromatography is a useful procedure, which can help in identifying carbohydrates. In this experiment Thin Layer Chromatography (TLC) will be used. All chromatographic systems have a stationary phase (in this case silica gel) and a mobile phase (in this case an organic mixture with some buffer). In this experiment you will separate two groups of four samples. You are asked to comment on the separation. The factors you should consider are structural i.e.: pentose, hexose, monosaccharide, and disaccharide. You will be using mini-plates, which only take about 20 minutes to develop. Method Using the side of the aluminium plate covered with silica gel, make a pencil mark about 1.5cm from the bottom of the plate, and label four sites to receive your samples. Do not handle the surface of the plate. The samples are applied using capillary tubes (one per sample). Allow the silica gel to draw...
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