Biology Coursework - Osmosis - To Investigate concentration of sucrose solution is isotonic to potato cell sap.

... Biology Coursework - Osmosis Planning Aim: To investigate what concentration of sucrose solution is isotonic to potato cell sap. Apparatus: 0M sucrose (aq) (distilled water) 2M sucrose (aq) Potato Chip cutter Knife Boiling tubes Measuring cylinder (25cm3) Vernier callipers (to nearest 0.1mm) Balance (to nearest 0.01gram) Safety: I will ensure safety around the lab by: wearing safety goggles, laying paper towels, removing baggage from walking space, push stools under the bench, stand up, walk carefully and slowly - don't rush and handle equipment and apparatus carefully. Preliminary Experiment: I did a small experiment before doing the real one so I could find a suitable range to base my investigation round. I used 3 chips in 3 boiling tubes, each containing 20cm3 of: 0M sucrose, 0.5M sucrose and 1M sucrose. Moles of Sucrose /M Original Mass /g Mass after 3 hours /g Up/Down in mass 0M 8.02 9.94 Up 0.5M 8.18 7.83 Down 1M 8.83 7.20 Down As you can see from my preliminary results, I should investigate between 0M sucrose and 0.5M sucrose as a suitable range. I predicted that the isotonic

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