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An Experiment Testing for Carbohydrates Non-Reducing sugars
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| Submitted: Thu Jul 11 2002
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An Experiment Testing for Carbohydrates Non-Reducing sugars 28 September 2001 Aim To test for non-reducing sugars in 4 known substances and 1 unknown substance using Benedict's reagent and Hydrochloric acid neutralised with Sodium Hydrogen Carbonate. Introduction Benedict's test uses copper (II) sulphate. This reagent is used as a general test for detecting reducing sugars. If the saccharide is a reducing sugar, it will reduce the copper (II) ions to copper (I) oxide, and form a red precipitate. However, some saccharides need to be split and neutralised in order to detect their reducing sugars. Risks Hydrochloric acid is very poisonous and corrosive if spilt wash affected area immediately. Apparatus and Materials Boiling tubes x 15, Pestle and mortar, Water bath, Stop clock, Permanent Marker, 8 pipettes 10cm³, 9cm³ 0.1% Glucose, 9cm³ 0.1% Sucrose, 9cm³ Unknown solution, 1g Grass, 1g Molasses, Litmus Paper, 20cm³ Water, 3cm³ Dilute Hydrochloric acid, 75cm³ Benedict's...
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