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A colorimetric method for the estimation of glucose in solution Aim: To find percentage of glucose in an unknown glucose solution. Apparatus; Unknown glucose solution, 1% glucose solution, colorimeter, cuvettes, water bath, test tube rack, test tube, distilled water. I will use 1% glucose solution to make up the dilution series as follows. Composition Tube % glucose 1% glucose (cm3) distilled water (cm3) 1 ---- 0 10 2 0.1 1 9 3 0.2 2 8 4 0.3 3 7 5 0.4 4 6 6 0.5 5 5 7 0.6 6 4 8 0.7 7 3 9 0.8 8 2 10 0.9 9 1 11 1 10 0 When making up the compositions of glucose, I will use a teat pipette to get the required amount of glucose/water and put them together in the test tube. I will then shake the test tube to mix up the liquids so that the concentration is the same through out the liquid When measuring out amounts of liquid I will make sure that the bottom of the meniscus of the circle of the top of the liquid is on the measuring line. I will use a 10cm3 pipette to put Benedict's reagent in to 13 boiling...

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