A Colorimetric Method for the Estimation of Glucose in Solution.

... A Colorimetric Method for the Estimation of Glucose in Solution Method A clean pipette was used to transfer 10cm of 10% glucose into a boiling tube. The pipette was then used to transfer 9cm of 10% glucose into a different boiling tube. 1 cm of distilled water was added to give 10cm of 9% glucose. This was repeated to make 10cm of 8%, 7%, 6%, 5%, 4%, 3%, 2%, and 1% glucose solutions (use the table below to help): Concentration of glucose (%) Volume of 10% glucose to transfer (cm ) Volume of distilled water to transfer (cm ) Total volume of solution (cm ) 10 10 0 10 9 9 1 10 8 8 2 10 7 7 3 10 6 6 4 10 5 5 5 10 4 4 6 10 3 3 7 10 2 2 8 10 1 1 9 10 A clean pipette was used to transfer 5 cm of 1M sulphuric acid into each solution. A clean pipette and pipette filler was used to transfer 2cm of potassium permanganate solution into each boiling tube. A stopwatch was started the exact moment the potassium permanganate was added to the acidified glucose solutions. A time was recorded, in seconds for the

want to read more? →