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STUDY AND LABORATORY SKILLS - SPECTROPHOTOMETRY Introduction Much of the background to spectrophotometry and ERE (electromagnetic radiant energy) phenomena has been discussed in the schedule. The schedule states that the energy associated with ERE is calculated by the formula E=hv This is infact the incorrect terming for 'Planck's Equation' and the more widely accepted version of: E=hf Where E is the energy in joules, h is Planck's constant (6.626x10-34 Js) and f is the frequency in hertz. The reagent used is cytochrome C, which is essentially a biological extract and not a synthetic product. Aims Again the aims are already stated in the schedule. However it is necessary jus to summarise them: Determine the peak wavelength of absorption of cyt c solution Produce a calibration curve for cyt c solution (using a range of different concentrations of cyt c) Determine the concentration of a solution of cyt c of an unknown concentration, from the...

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