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Seperation of Chlorophyll pigments by paper chromatography  

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SEPERATION OF CHLOROPHYLL PIGMENTS BY PAPER CHROMATOGRAPHY Results of experiment Rf value for 1 = 2.6 / 7 = 0.37 Rf value for 2 = 3.2 / 7 = 0.45 Rf value for 3 = 5.6 / 7 = 0.80 Analysis From looking at the table of Rf values I have definitely found chlorophyll b and xanthophylls. But there were three different pigments with distinct colour differences, and from previous knowledge of the colours of photosynthetic pigments, I know that chlorophyll a is the other one. Chlorophyll a is the fundamental pigment in plants and without it they would not be able to photosynthesise. Paper chromatography separates the mixture of pigments (in liquid form) into its individual components. The technique is based on the fact that each substance in the mixture has a specific affinity for a solid surface and a specific solubility in different solvents. By this method, the solid surface is the cellulose fibres in...

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